Transcriptome Intelligence
Choose the molecular strategy that fits your research goals. From targeted mRNA to the full spectrum of non-coding noise.
mRNA-Seq (PolyA)
Focusing exclusively on the coding transcriptome by enriching for transcripts with polyadenylated tails.
Total RNA-Seq
Ribosomal RNA depletion to capture both coding (mRNA) and non-coding RNAs (lncRNA, snoRNA).
Small RNA-Seq
Targeting microRNAs and other small RNAs (<200nt) involved in gene regulation and silencing.
Expression
Gold Standards
Our RNA-Seq pipelines are optimized for high sensitivity and wide dynamic range. By utilizing stranded library prep and deep sequencing, we capture rare transcripts that microarrays miss.
- Stranded (Sense-Specific) Library Protocol
- Detection of novel splice junctions & fusions
- High-confidence quantification of low-abundance genes
- Comprehensive pathway & network analysis
Key Applications
Disease Biomarkers
Identify novel transcriptomic signatures and diagnostic biomarkers in oncology, immunology, and metabolic disorders.
Drug Discovery
Assess drug efficacy and toxicity by analyzing global gene expression changes in response to therapeutic compounds.
Agricultural Genomics
Study plant responses to biotic and abiotic stress to engineer resilient, high-yield crop varieties.
Microbial Transcriptomics
Unravel pathogen-host interactions and map regulatory networks in complex microbial communities.
From Discovery to Validation
RNA-Seq is the workhorse of modern biology. Whether you are performing biomarker discovery in oncology or investigating plant stress responses, our data provides actionable biological insights.
Differential Expression
Robust statistical modeling to find genes shifting across experimental conditions.
Isoform Characterization
Resolving the complexity of alternative splicing and transcript diversity.
The RNA Command
RNA Extraction
Specialized RNA isolation prioritizing RNA Integrity Number (RIN) > 7 for optimal library preparation.
Library Prep
Poly-A selection or rRNA depletion followed by stranded cDNA synthesis and adapter ligation.
Sequencing
Illumina NovaSeq X Plus paired-end sequencing targeting 30-50 million reads per sample.
Alignment & QC
Stringent quality filtering and splice-aware alignment (STAR/HISAT2) to the reference genome.
Expression Analysis
Differential Gene Expression (DGE) modeling, pathway enrichment (GO/KEGG), and data visualization.
Supported Sample Types & Requirements
Client Deliverables
Technical FAQ
Decode the Dynamic State.
Don't just count reads. Gain deep regulatory insights with our multi-dimensional RNA-Seq pipelines.