Next Generation Sequencing
Ponnaiya CAG provides next-generation sequencing services, delivering precise and effective analysis of genomic data to propel advancements in both research and clinical applications.
Whole Genome Sequencing and Data Analysis
Unlock the mysteries of your genome with our comprehensive Whole Genome Sequencing (WGS) and Data Analysis services. Our cutting-edge technologies and expert bioinformatics analysis provide you with invaluable insights into your genetic makeup, enabling personalized healthcare and groundbreaking discoveries. Using state-of-the-art next-generation sequencing (NGS) platforms, we accurately sequence all 3 billion base pairs of your genome.
Key Features:
High-throughput sequencing for accurate and efficient genome coverage.
Detection of single nucleotide polymorphisms (SNPs), insertions, deletions, and structural variations.
Identification of disease-causing mutations and genetic predispositions.
Annotation of genetic variants with functional implications.
Variant calling and annotation to identify genetic variations.
Functional analysis to assess the impact of variants on gene function.
Population genetics analysis for understanding genetic diversity and ancestry.
Pharmacogenomics analysis to predict drug responses based on genetic variations.
Disease association studies to uncover genetic factors underlying complex diseases.
Whole Exome Sequencing and Data Analysis
Uncover the secrets of the exome with our comprehensive Whole Exome Sequencing (WES) and Data Analysis services. Our advanced technologies and expert bioinformatics analysis provide you with valuable insights into the protein-coding regions of your genome, enabling precise diagnosis, personalized medicine, and groundbreaking research. By capturing and sequencing these critical regions, we provide a cost-effective approach to identify disease-causing variants and genetic predispositions.
Key Features
Comprehensive coverage of over 20,000 protein-coding genes.
Targeted capture and enrichment of exonic regions using state-of-the-art technologies.
High-throughput sequencing for accurate and efficient exome coverage.
Detection of single nucleotide variants (SNVs), insertions, deletions, and structural variations.
Variant calling and annotation to identify genetic variations within the exome.
Functional analysis to assess the impact of variants on gene function and protein structure.
Prioritization of disease-causing variants using population databases and in-silico prediction tools.
Interpretation of results in the context of clinical relevance and disease association studies.
RNA Sequencing and Data Analysis
Our RNA Sequencing service offers a comprehensive analysis of the transcriptome, providing a snapshot of all RNA molecules present in a biological sample. Using state-of-the-art next-generation sequencing (NGS) platforms, we accurately sequence and quantify RNA transcripts, including messenger RNA (mRNA), non-coding RNA (ncRNA), and splice variants.
Key Features
Transcriptome-wide coverage of gene expression levels.
Detection and quantification of different RNA species, including mRNA, microRNA (miRNA), long non-coding RNA (lncRNA), and circular RNA (circRNA).
Differential gene expression analysis to identify genes that are upregulated or downregulated under different experimental conditions.
Alternative splicing analysis to uncover isoform diversity and transcriptomic complexity.
Novel transcript discovery for the identification of previously unknown RNA transcripts.
Data Analysis:
Interpreting the vast amount of data generated by RNA sequencing requires sophisticated bioinformatics analysis. Our Data Analysis services leverage cutting-edge algorithms and computational methods to extract meaningful information from raw sequencing data.
Quality control and preprocessing of raw sequencing reads.
Alignment of reads to a reference genome or transcriptome.
Quantification of gene expression levels and differential expression analysis.
Functional annotation and pathway analysis to elucidate biological functions and pathways associated with differentially expressed genes.
Identification of alternative splicing events and transcript isoforms.
Prediction of novel transcripts and non-coding RNA molecules.
Integration with other omics data (e.g., proteomics, metabolomics) for comprehensive systems biology analysis.
Meta-Genomics
Explore the hidden world of microbial communities with our comprehensive Metagenomic Sequencing and Data Analysis services. Our advanced technologies and expert bioinformatics analysis provide you with invaluable insights into the composition, diversity, and functional potential of microbial ecosystems, enabling groundbreaking discoveries in environmental microbiology, human health, agriculture, and beyond. Our Metagenomic Sequencing service offers a comprehensive analysis of microbial communities present in complex samples, such as soil, water, the human gut, or clinical specimens. Using state-of-the-art next-generation sequencing (NGS) platforms, we accurately sequence and characterize the genetic material of all microorganisms present in a sample.
Key Features
Whole-community DNA sequencing to capture the entire genetic diversity of microbial populations.
Identification of bacteria, archaea, fungi, viruses, and other microorganisms.
Quantification of microbial abundance and diversity within a sample.
Functional profiling to assess the metabolic potential and functional capabilities of microbial communities.
Characterization of mobile genetic elements, antibiotic resistance genes, and virulence factors.
Taxonomic classification of microbial sequences to identify the organisms present in the sample.
Phylogenetic analysis to assess evolutionary relationships between microbial taxa.
Functional annotation to predict the metabolic pathways and biological functions encoded by microbial genes.
Comparative analysis to identify differences in microbial composition and function between samples.
Biomarker discovery for identifying microbial signatures associated with specific environmental conditions or disease states.
Why Choose Us?
Expertise: Our team of bioinformaticians and geneticists are experts in WGS, WES, RNA-Seq, 16s sequencing and data analysis, ensuring accurate and reliable results.
State-of-the-Art Infrastructure: We utilize the latest sequencing technologies and bioinformatics tools to deliver high-quality genomic data.
Customized Solutions: We tailor our services to meet your specific research or clinical needs, providing flexible and customizable solutions.
Data Security: We prioritize the confidentiality and security of your genomic data, adhering to strict data protection protocols.